Micropropagation of dwarf banana (Musa spp) using hands of immature male flowers with different concentrations of disinfectants and growth regulators

Abstract

The plantain is a food industry that has a great economic importance in our country; it is considered as a national high consumed food. One of the fundamental purposes of the in vitro farming applied to agriculture – is to increase the production and / or the performance of this industry through the new approaches, allowing the expansion of the sown field. The main objective of this research was to establish a protocol of micro propagation of the Dwarf Cavendish Banana using handful immature male flowers with the purpose of reducing the in vitro production costs

Initially, this research was going to be carried out in two stages, but at the end it was done only the in vitro stage (superficial disinfection) because it was determined from Musa spp (Dwarf Cavendish banana) that it was not possible to get plants through the male inflorescence. This was because, somehow, that the disinfected agents did not allow to get explant free from pollutant pathogens; in addition to the action of the polyphenol oxidases, that did not show the potentiality of the explants used for the development of the embryogenic calli.